The clip of the prairie dog is from the Japanese television show Hello! Morning featuring Mini Moni. The clip has a prairie dog inside a transparent box being shown to the hosts in the studio. CollegeHumor also released a longer clip under the title Undramatic Chipmunk, showing how the video looked in the original Japanese version.
The music used in Dramatic Chipmunk is taken from the score of the 1974 film Young Frankenstein, and was composed by John Morris. It uses three dramatic chords, and has a rumble of thunder in the background. The "chipmunk" turns its head and stares at the camera while the music is played.
Since its release, the video has received tens of millions of views. People Magazine named the Dramatic Chipmunk as one of The 10 Wildest YouTube Stars of 2007.